Hotspot

ccsA

SSC · 105828-106787 · 960 bp

Pi 0.00254

MarkerSeek score 29.80

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 960
variable_sites: 12
pi: 0.00254
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTCAAGCATCTCGGTTCGAA	TGAGTGGTTTTGTCGCAGAA	50.0	45.0	58.0	58.0	1570-1574 bp	1.0	81.1
ccsA_p2	CTCAAGCATCTCGGTTCGAA	GCCTTACCAGGAATGAGTGG	50.0	55.0	58.0	58.0	1583-1587 bp	1.0	81.0
ccsA_p3	CTCAAGCATCTCGGTTCGAA	ATCTCGAAGAACTTGGGGGA	50.0	50.0	58.0	58.0	1663-1667 bp	1.0	80.9
ccsA_p4	AAGCATCTCGGTTCGAATCC	TGAGTGGTTTTGTCGCAGAA	50.0	45.0	58.1	58.0	1567-1571 bp	1.0	80.7
ccsA_p5	AAGCATCTCGGTTCGAATCC	GCCTTACCAGGAATGAGTGG	50.0	55.0	58.1	58.0	1580-1584 bp	1.0	80.5