Hotspot

psbE-petL

LSC · 61803-63074 · 1272 bp

Back to Avena

Pi 0.00234
MarkerSeek score 47.50
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1272
variable_sites
19
pi
0.00234
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TGACAAATAACCAACCCGCA GCCAATAAATAGAGCTGGGGT 45.0 47.6 58.0 57.8 1449-1460 bp 1.0 71.6
psbE-petL_p2 CAACCCGCAATGAATAGGGA GCCAATAAATAGAGCTGGGGT 50.0 47.6 57.9 57.8 1438-1449 bp 1.0 71.4
psbE-petL_p3 ACCAACCCGCAATGAATAGG GCCAATAAATAGAGCTGGGGT 50.0 47.6 58.2 57.8 1440-1451 bp 1.0 69.9
psbE-petL_p4 TGACAAATAACCAACCCGCA AGCCAATAAATAGAGCTGGGG 45.0 47.6 58.0 57.5 1450-1461 bp 1.0 69.3
psbE-petL_p5 CAACCCGCAATGAATAGGGA AGCCAATAAATAGAGCTGGGG 50.0 47.6 57.9 57.5 1439-1450 bp 1.0 69.1