Hotspot

trnD-GUC-psbM

LSC · 15897-16596 · 700 bp

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Pi 0.00434
MarkerSeek score 50.10
Primer available yes
Label trnD-GUC-psbM

Feature Metadata

label_name
trnD-GUC-psbM
feature_type
igs
region
LSC
length_bp
700
variable_sites
16
pi
0.00434
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-psbM_p1 TTCAATTGGTCAGAGCACCG AGAATGAACAGTGCAGTAGC 50.0 45.0 58.5 55.8 611-827 bp 1.0 63.9
trnD-GUC-psbM_p2 TTCAATTGGTCAGAGCACCG AGGAACTAGAATGAACAGTGCA 50.0 40.9 58.5 57.4 618-834 bp 1.0 60.8
trnD-GUC-psbM_p3 GTTCAATTGGTCAGAGCACC AGAATGAACAGTGCAGTAGC 50.0 45.0 57.0 55.8 612-828 bp 1.0 59.8
trnD-GUC-psbM_p4 CGCGGGGATTGTAGTTCAA AGAATGAACAGTGCAGTAGC 52.6 45.0 57.9 55.8 625-841 bp 1.0 58.6
trnD-GUC-psbM_p5 GGTATGCCATACATCCCGC AGAATGAACAGTGCAGTAGC 57.9 45.0 58.1 55.8 641-857 bp 1.0 58.5