Hotspot

trnY-GUA-trnD-GUC

LSC · 15508-15822 · 315 bp

Back to Avena

Pi 0.00526
MarkerSeek score 44.80
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
315
variable_sites
11
pi
0.00526
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 AATTCTTCCTGGGTCGATGC CGGTGCTCTGACCAATTGAA 50.0 50.0 58.0 58.5 434-439 bp 1.0 78.7
trnY-GUA-trnD-GUC_p2 GGGGACGGACTGTAAATTCG CGGTGCTCTGACCAATTGAA 55.0 50.0 58.4 58.5 401-406 bp 1.0 76.5
trnY-GUA-trnD-GUC_p3 GGGACGGACTGTAAATTCGT CGGTGCTCTGACCAATTGAA 50.0 50.0 57.6 58.5 400-405 bp 1.0 76.3
trnY-GUA-trnD-GUC_p4 AATTCTTCCTGGGTCGATGC GGTGCTCTGACCAATTGAAC 50.0 50.0 58.0 57.0 433-438 bp 1.0 74.6
trnY-GUA-trnD-GUC_p5 TGGGGACGGACTGTAAATTC CGGTGCTCTGACCAATTGAA 50.0 50.0 57.2 58.5 402-407 bp 1.0 73.1