Hotspot

rps12a-trnT-GGU

LSC · 14306-14683 · 378 bp

Back to Avena

Pi 0.00463
MarkerSeek score 46.50
Primer available yes
Label rps12a-trnT-GGU

Feature Metadata

label_name
rps12a-trnT-GGU
feature_type
igs
region
LSC
length_bp
378
variable_sites
10
pi
0.00463
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12a-trnT-GGU_p1 ACCAAATGTTCCTTCCACGA CGTCTTAGCACGGCATTACT 45.0 50.0 57.4 58.1 543 bp 0.925926 72.5
rps12a-trnT-GGU_p2 ACCAAATGTTCCTTCCACGA CTTACGTCTTAGCACGGCAT 45.0 50.0 57.4 58.1 547 bp 0.925926 72.5
rps12a-trnT-GGU_p3 ACCAAATGTTCCTTCCACGA ACGTCTTAGCACGGCATTAC 45.0 50.0 57.4 58.4 544 bp 0.925926 70.3
rps12a-trnT-GGU_p4 ACCAAATGTTCCTTCCACGA TTACGTCTTAGCACGGCATT 45.0 45.0 57.4 57.6 546 bp 0.925926 70.2
rps12a-trnT-GGU_p5 ACCAAATGTTCCTTCCACGAA CGTCTTAGCACGGCATTACT 42.9 50.0 58.1 58.1 543 bp 0.925926 69.3