Hotspot

trnG-UCC-trnI

LSC · 13512-14193 · 682 bp

Back to Avena

Pi 0.00433
MarkerSeek score 53.10
Primer available yes
Label trnG-UCC-trnI

Feature Metadata

label_name
trnG-UCC-trnI
feature_type
igs
region
LSC
length_bp
682
variable_sites
17
pi
0.00433
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnI_p1 ACGAATCACACTTTTACCACT GCGGATTCTGGAACCCTAAG 38.1 55.0 55.5 58.0 832-840 bp 0.925926 51.0
trnG-UCC-trnI_p2 AGAACGAATCACACTTTTACCAC GCGGATTCTGGAACCCTAAG 39.1 55.0 57.3 58.0 835-843 bp 0.925926 49.9
trnG-UCC-trnI_p3 GAACGAATCACACTTTTACCACT GCGGATTCTGGAACCCTAAG 39.1 55.0 57.3 58.0 834-842 bp 0.925926 49.9
trnG-UCC-trnI_p4 AACGAATCACACTTTTACCACT GCGGATTCTGGAACCCTAAG 36.4 55.0 56.2 58.0 833-841 bp 0.925926 49.1
trnG-UCC-trnI_p5 TACCACTAAACTATACCCGC GCGGATTCTGGAACCCTAAG 45.0 55.0 54.0 58.0 818-826 bp 0.925926 47.2