Hotspot

psbK-psbI

LSC · 6937-7344 · 408 bp

Back to Avena

Pi 0.00558
MarkerSeek score 46.30
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
408
variable_sites
12
pi
0.00558
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG GATTCCGTCCTGGGTCATTG 50.0 55.0 57.9 58.3 521-533 bp 1.0 80.4
psbK-psbI_p2 CGTGGATGTTATGCCTGTCA GATTCCGTCCTGGGTCATTG 50.0 55.0 58.0 58.3 570-582 bp 1.0 80.3
psbK-psbI_p3 CGTGGATGTTATGCCTGTCA GAATTTTTACTCCTCGCGCC 50.0 50.0 58.0 57.5 593-605 bp 1.0 79.2
psbK-psbI_p4 CGTGGATGTTATGCCTGTCA GGAATTTTTACTCCTCGCGC 50.0 50.0 58.0 57.5 594-606 bp 1.0 79.2
psbK-psbI_p5 GTTTGGCAAGCTGCTGTAAG GAATTTTTACTCCTCGCGCC 50.0 50.0 57.9 57.5 544-556 bp 1.0 79.2