Hotspot

rps16-trnQ-UUG

LSC · 5540-6329 · 790 bp

Back to Avena

Pi 0.00415
MarkerSeek score 49.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
790
variable_sites
14
pi
0.00415
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 852-890 bp 1.0 81.2
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 863-901 bp 1.0 80.6
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA AGTGTCGCATAAAACCCGAA 45.0 45.0 58.1 57.8 921-959 bp 1.0 80.2
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 856-894 bp 1.0 80.1
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC AGTGTCGCATAAAACCCGAA 50.0 45.0 58.2 57.8 932-970 bp 1.0 79.7