primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	5540	6329	852-890	1.000000	81.2
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	5540	6329	863-901	1.000000	80.6
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	AGTGTCGCATAAAACCCGAA	20	20	45.0	45.0	58.1	57.8	8.1	0.0	0.312175	5540	6329	921-959	1.000000	80.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	5540	6329	856-894	1.000000	80.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCATGTCCTTCAAGTCGCAC	AGTGTCGCATAAAACCCGAA	20	20	50.0	45.0	58.2	57.8	0.0	0.0	0.377084	5540	6329	932-970	1.000000	79.7
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	GATTCCGTCCTGGGTCATTG	20	20	50.0	55.0	57.9	58.3	1.4	0.0	0.387675	6937	7344	521-533	1.000000	80.4
psbK-psbI_p2	psbK-psbI	2	CGTGGATGTTATGCCTGTCA	GATTCCGTCCTGGGTCATTG	20	20	50.0	55.0	58.0	58.3	0.0	0.0	0.354345	6937	7344	570-582	1.000000	80.3
psbK-psbI_p3	psbK-psbI	3	CGTGGATGTTATGCCTGTCA	GAATTTTTACTCCTCGCGCC	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.476414	6937	7344	593-605	1.000000	79.2
psbK-psbI_p4	psbK-psbI	4	CGTGGATGTTATGCCTGTCA	GGAATTTTTACTCCTCGCGC	20	20	50.0	50.0	58.0	57.5	0.0	0.0	0.476414	6937	7344	594-606	1.000000	79.2
psbK-psbI_p5	psbK-psbI	5	GTTTGGCAAGCTGCTGTAAG	GAATTTTTACTCCTCGCGCC	20	20	50.0	50.0	57.9	57.5	1.4	0.0	0.509744	6937	7344	544-556	1.000000	79.2
trnG-UCC-trnI_p1	trnG-UCC-trnI	1	ACGAATCACACTTTTACCACT	GCGGATTCTGGAACCCTAAG	21	20	38.1	55.0	55.5	58.0	0.0	0.0	3.580916	13512	14193	832-840	0.925926	51.0
trnG-UCC-trnI_p2	trnG-UCC-trnI	2	AGAACGAATCACACTTTTACCAC	GCGGATTCTGGAACCCTAAG	23	20	39.1	55.0	57.3	58.0	0.0	0.0	3.713631	13512	14193	835-843	0.925926	49.9
trnG-UCC-trnI_p3	trnG-UCC-trnI	3	GAACGAATCACACTTTTACCACT	GCGGATTCTGGAACCCTAAG	23	20	39.1	55.0	57.3	58.0	0.0	0.0	3.713631	13512	14193	834-842	0.925926	49.9
trnG-UCC-trnI_p4	trnG-UCC-trnI	4	AACGAATCACACTTTTACCACT	GCGGATTCTGGAACCCTAAG	22	20	36.4	55.0	56.2	58.0	0.0	0.0	3.821386	13512	14193	833-841	0.925926	49.1
trnG-UCC-trnI_p5	trnG-UCC-trnI	5	TACCACTAAACTATACCCGC	GCGGATTCTGGAACCCTAAG	20	20	45.0	55.0	54.0	58.0	0.0	0.0	4.056149	13512	14193	818-826	0.925926	47.2
rps12a-trnT-GGU_p1	rps12a-trnT-GGU	1	ACCAAATGTTCCTTCCACGA	CGTCTTAGCACGGCATTACT	20	20	45.0	50.0	57.4	58.1	0.0	0.0	0.726961	14306	14683	543-543	0.925926	72.5
rps12a-trnT-GGU_p2	rps12a-trnT-GGU	2	ACCAAATGTTCCTTCCACGA	CTTACGTCTTAGCACGGCAT	20	20	45.0	50.0	57.4	58.1	0.0	0.0	0.726961	14306	14683	547-547	0.925926	72.5
rps12a-trnT-GGU_p3	rps12a-trnT-GGU	3	ACCAAATGTTCCTTCCACGA	ACGTCTTAGCACGGCATTAC	20	20	45.0	50.0	57.4	58.4	0.0	8.5	1.012543	14306	14683	544-544	0.925926	70.3
rps12a-trnT-GGU_p4	rps12a-trnT-GGU	4	ACCAAATGTTCCTTCCACGA	TTACGTCTTAGCACGGCATT	20	20	45.0	45.0	57.4	57.6	0.0	0.0	1.023072	14306	14683	546-546	0.925926	70.2
rps12a-trnT-GGU_p5	rps12a-trnT-GGU	5	ACCAAATGTTCCTTCCACGAA	CGTCTTAGCACGGCATTACT	21	20	42.9	50.0	58.1	58.1	0.0	0.0	1.138592	14306	14683	543-543	0.925926	69.3
trnY-GUA-trnD-GUC_p1	trnY-GUA-trnD-GUC	1	AATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	58.0	58.5	0.0	7.1	0.521454	15508	15822	434-439	1.000000	78.7
trnY-GUA-trnD-GUC_p2	trnY-GUA-trnD-GUC	2	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.828828	15508	15822	401-406	1.000000	76.5
trnY-GUA-trnD-GUC_p3	trnY-GUA-trnD-GUC	3	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.6	58.5	0.0	7.1	0.852505	15508	15822	400-405	1.000000	76.3
trnY-GUA-trnD-GUC_p4	trnY-GUA-trnD-GUC	4	AATTCTTCCTGGGTCGATGC	GGTGCTCTGACCAATTGAAC	20	20	50.0	50.0	58.0	57.0	0.0	7.1	1.041608	15508	15822	433-438	1.000000	74.6
trnY-GUA-trnD-GUC_p5	trnY-GUA-trnD-GUC	5	TGGGGACGGACTGTAAATTC	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.2	58.5	0.0	7.1	1.251324	15508	15822	402-407	1.000000	73.1
trnD-GUC-psbM_p1	trnD-GUC-psbM	1	TTCAATTGGTCAGAGCACCG	AGAATGAACAGTGCAGTAGC	20	20	50.0	45.0	58.5	55.8	5.4	0.0	2.706630	15897	16596	611-827	1.000000	63.9
trnD-GUC-psbM_p2	trnD-GUC-psbM	2	TTCAATTGGTCAGAGCACCG	AGGAACTAGAATGAACAGTGCA	20	22	50.0	40.9	58.5	57.4	5.4	0.0	3.101264	15897	16596	618-834	1.000000	60.8
trnD-GUC-psbM_p3	trnD-GUC-psbM	3	GTTCAATTGGTCAGAGCACC	AGAATGAACAGTGCAGTAGC	20	20	50.0	45.0	57.0	55.8	5.4	0.0	3.226784	15897	16596	612-828	1.000000	59.8
trnD-GUC-psbM_p4	trnD-GUC-psbM	4	CGCGGGGATTGTAGTTCAA	AGAATGAACAGTGCAGTAGC	19	20	52.6	45.0	57.9	55.8	0.0	0.0	3.376082	15897	16596	625-841	1.000000	58.6
trnD-GUC-psbM_p5	trnD-GUC-psbM	5	GGTATGCCATACATCCCGC	AGAATGAACAGTGCAGTAGC	19	20	57.9	45.0	58.1	55.8	16.3	0.0	3.374776	15897	16596	641-857	1.000000	58.5
trnR-UCU-trnfM_p1	trnR-UCU-trnfM	1	AGAAGACCTCTGTCCTATCCA	CAGGTTCGATTCCCACTACC	21	20	47.6	55.0	56.9	57.7	0.0	3.9	2.362729	35428	35541	175-180	1.000000	64.3
trnR-UCU-trnfM_p2	trnR-UCU-trnfM	2	AGAAGACCTCTGTCCTATCCA	CTTGGTAGCTCACAAGGCTC	21	20	47.6	55.0	56.9	58.3	0.0	18.0	2.319885	35428	35541	210-215	1.000000	64.1
trnR-UCU-trnfM_p3	trnR-UCU-trnfM	3	AGAAGACCTCTGTCCTATCCA	GCAGGTTCGATTCCCACTAC	21	20	47.6	55.0	56.9	58.3	0.0	3.9	2.398453	35428	35541	176-181	1.000000	64.0
trnR-UCU-trnfM_p4	trnR-UCU-trnfM	4	AGGTTTAGAAGACCTCTGTCCT	CAGGTTCGATTCCCACTACC	22	20	45.5	55.0	57.9	57.7	0.0	3.9	2.446057	35428	35541	181-186	1.000000	63.5
trnR-UCU-trnfM_p5	trnR-UCU-trnfM	5	AGGTTTAGAAGACCTCTGTCCT	CTTGGTAGCTCACAAGGCTC	22	20	45.5	55.0	57.9	58.3	0.0	18.0	2.403213	35428	35541	216-221	1.000000	63.4
psaA-ycf3_p1	psaA-ycf3	1	GGTCTGGCCCATTCTTCAAA	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.132613	40725	41367	800-819	1.000000	80.5
psaA-ycf3_p2	psaA-ycf3	2	GGTCTGGCCCATTCTTCAAA	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	58.1	57.7	0.0	19.9	0.351778	40725	41367	839-858	1.000000	78.7
psaA-ycf3_p3	psaA-ycf3	3	GGTCTGGCCCATTCTTCAAA	GGTTGAAGATTACGAAGCGC	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.513952	40725	41367	763-782	1.000000	77.6
psaA-ycf3_p4	psaA-ycf3	4	TGTTCTTGAGAAATGGCCGG	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.522343	40725	41367	819-838	1.000000	77.4
psaA-ycf3_p5	psaA-ycf3	5	TTTTACTTCTGGTTCCGGCG	GCGCTTACTCCGGGAAATTA	20	20	50.0	50.0	58.5	58.1	0.0	0.0	0.540887	40725	41367	747-766	1.000000	77.4
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	ATTTGAACCGATGACCCTCG	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.032006	45348	46161	723-917	1.000000	85.3
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	CGATGACCCTCGCATTACAA	ACAATCAAGTCCGTAGCGTC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.073242	45348	46161	715-909	1.000000	85.0
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	ACAATCAAGTCCGTAGCGTC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.140570	45348	46161	693-887	1.000000	84.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	ATTTGAACCGATGACCCTCG	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	58.0	58.2	0.0	10.2	0.272624	45348	46161	713-907	1.000000	83.4
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ATTTGAACCGATGACCCTCG	CAATCAAGTCCGTAGCGTCT	20	20	50.0	50.0	58.0	57.7	0.0	0.0	0.303758	45348	46161	722-916	1.000000	83.2
rpl23_p1	rpl23	1	GCCTGTGTACAAGCTCGTAA	GCGAAGTCGTCCTGTTATGT	20	20	50.0	50.0	57.9	58.0	0.0	2.3	0.082377	55651	55929	1275-1282	1.000000	81.7
rpl23_p2	rpl23	2	GTAAGCTAGAAGGGGAACGC	GCGAAGTCGTCCTGTTATGT	20	20	55.0	50.0	58.1	58.0	0.0	2.3	0.070552	55651	55929	1553-1560	1.000000	81.4
rpl23_p3	rpl23	3	TGCTCGCGGTATCTTTTTCA	GCGAAGTCGTCCTGTTATGT	20	20	45.0	50.0	57.9	58.0	6.1	2.3	0.095331	55651	55929	1477-1484	1.000000	81.3
rpl23_p4	rpl23	4	TGCCAGCTCTGACTGAAATC	GCGAAGTCGTCCTGTTATGT	20	20	50.0	50.0	57.9	58.0	0.0	2.3	0.112836	55651	55929	1391-1398	1.000000	81.3
rpl23_p5	rpl23	5	GTAAGCTAGAAGGGGAACGC	AAGCATTGTATCGTGGGGTC	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.101109	55651	55929	1413-1417	0.777778	71.2
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTCGC	TCCTCTTTGGCTGATAGGTACT	20	22	50.0	45.5	57.9	58.0	0.0	0.0	2.056243	60341	61033	890-898	1.000000	65.8
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	CCTCTTTGGCTGATAGGTACTG	20	22	50.0	50.0	57.9	57.9	0.0	0.0	2.117200	60341	61033	889-897	1.000000	65.3
petA-psbJ_p3	petA-psbJ	3	TGTTCTTCTTCGCATCCGTT	TCCTCTTTGGCTGATAGGTACT	20	22	45.0	45.5	57.8	58.0	0.0	0.0	2.168182	60341	61033	883-891	1.000000	64.9
petA-psbJ_p4	petA-psbJ	4	TTGTTCTTCTTCGCATCCGT	TCCTCTTTGGCTGATAGGTACT	20	22	45.0	45.5	57.8	58.0	0.0	0.0	2.168182	60341	61033	884-892	1.000000	64.9
petA-psbJ_p5	petA-psbJ	5	TGTTCTTCTTCGCATCCGTT	CCTCTTTGGCTGATAGGTACTG	20	22	45.0	50.0	57.8	57.9	0.0	0.0	2.229139	60341	61033	882-890	1.000000	64.4
psbE-petL_p1	psbE-petL	1	TGACAAATAACCAACCCGCA	GCCAATAAATAGAGCTGGGGT	20	21	45.0	47.6	58.0	57.8	0.0	0.0	1.247037	61803	63074	1449-1460	1.000000	71.6
psbE-petL_p2	psbE-petL	2	CAACCCGCAATGAATAGGGA	GCCAATAAATAGAGCTGGGGT	20	21	50.0	47.6	57.9	57.8	6.4	0.0	1.281031	61803	63074	1438-1449	1.000000	71.4
psbE-petL_p3	psbE-petL	3	ACCAACCCGCAATGAATAGG	GCCAATAAATAGAGCTGGGGT	20	21	50.0	47.6	58.2	57.8	0.0	0.0	1.466989	61803	63074	1440-1451	1.000000	69.9
psbE-petL_p4	psbE-petL	4	TGACAAATAACCAACCCGCA	AGCCAATAAATAGAGCTGGGG	20	21	45.0	47.6	58.0	57.5	0.0	0.0	1.532941	61803	63074	1450-1461	1.000000	69.3
psbE-petL_p5	psbE-petL	5	CAACCCGCAATGAATAGGGA	AGCCAATAAATAGAGCTGGGG	20	21	50.0	47.6	57.9	57.5	6.4	0.0	1.566936	61803	63074	1439-1450	1.000000	69.1
petL-petG_p1	petL-petG	1	ACCCCAGCTCTATTTATTGGC	TGCAGTCACGAATAATCCCG	21	20	47.6	50.0	57.8	58.1	0.0	0.0	1.295108	63171	63346	295-297	1.000000	71.3
petL-petG_p2	petL-petG	2	ACCCCAGCTCTATTTATTGGC	CAGTCACGAATAATCCCGCT	21	20	47.6	50.0	57.8	57.8	0.0	0.0	1.454765	63171	63346	293-295	1.000000	70.0
petL-petG_p3	petL-petG	3	CCCCAGCTCTATTTATTGGCT	TGCAGTCACGAATAATCCCG	21	20	47.6	50.0	57.5	58.1	0.0	0.0	1.581013	63171	63346	294-296	1.000000	69.0
petL-petG_p4	petL-petG	4	TGGCTTGAACAAGATACGTCT	TGCAGTCACGAATAATCCCG	21	20	42.9	50.0	57.3	58.1	17.2	0.0	1.743468	63171	63346	278-280	1.000000	67.8
petL-petG_p5	petL-petG	5	CCCCAGCTCTATTTATTGGCT	CAGTCACGAATAATCCCGCT	21	20	47.6	50.0	57.5	57.8	0.0	0.0	1.740670	63171	63346	292-294	1.000000	67.7
rpl16_p1	rpl16	1	TTATAACCGCTGCGTTGTCA	CTTCGCATTCTGTCCAGTGA	20	20	45.0	50.0	57.9	57.9	21.6	0.0	0.166335	77235	78693	1973-1980	1.000000	80.6
rpl16_p2	rpl16	2	TTATAACCGCTGCGTTGTCA	TCTTCGCATTCTGTCCAGTG	20	20	45.0	50.0	57.9	57.9	21.6	0.0	0.166335	77235	78693	1974-1981	1.000000	80.6
rpl16_p3	rpl16	3	TCGGATCTTTCTAGGGGCAT	CTTCGCATTCTGTCCAGTGA	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.187763	77235	78693	1881-1888	1.000000	80.5
rpl16_p4	rpl16	4	TCGGATCTTTCTAGGGGCAT	TCTTCGCATTCTGTCCAGTG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.187763	77235	78693	1882-1889	1.000000	80.5
rpl16_p5	rpl16	5	TTCGGATCTTTCTAGGGGCA	CTTCGCATTCTGTCCAGTGA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.210480	77235	78693	1882-1889	1.000000	80.3
ndhF-rpl32_p1	ndhF-rpl32	1	CCCATGCATATTGATATATATGTTCCA	TCCAAATATTTTTACGAATACGCT	27	24	33.3	29.2	57.4	55.3	0.0	0.0	14.296720	104003	104850	820-974	1.000000	46.2
ndhF-rpl32_p2	ndhF-rpl32	2	ACCCATGCATATTGATATATATGTTCC	TCCAAATATTTTTACGAATACGCT	27	24	33.3	29.2	57.4	55.3	0.0	0.0	14.298074	104003	104850	821-975	1.000000	46.2
ndhF-rpl32_p3	ndhF-rpl32	3	CCCATGCATATTGATATATATGTTCCA	TCCAAATATTTTTACGAATACGCTT	27	25	33.3	28.0	57.4	56.0	0.0	0.0	14.621871	104003	104850	820-974	1.000000	46.2
ndhF-rpl32_p4	ndhF-rpl32	4	CCCATGCATATTGATATATATGTTCCA	TTCCAAATATTTTTACGAATACGCT	27	25	33.3	28.0	57.4	56.0	0.0	0.0	14.621871	104003	104850	821-975	1.000000	46.2
ndhF-rpl32_p5	ndhF-rpl32	5	ACCCATGCATATTGATATATATGTTCC	TCCAAATATTTTTACGAATACGCTT	27	25	33.3	28.0	57.4	56.0	0.0	0.0	14.623226	104003	104850	821-975	1.000000	46.2
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	GGGGCAACAACAACAAACAA	TTCGAACCGAGATGCTTGAG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.104313	105040	105670	728-735	0.888889	76.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	GGGGCAACAACAACAAACAA	GGATTCGAACCGAGATGCTT	20	20	45.0	50.0	57.9	58.1	0.0	0.0	0.159280	105040	105670	731-738	0.888889	76.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	GGGGCAACAACAACAAACAA	TCCTAAGAGCAGCGTGTCTA	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.224882	105040	105670	701-708	0.888889	75.8
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	GGGGCAACAACAACAAACAA	AGCAGCGTGTCTACCAATTT	20	20	45.0	45.0	57.9	57.8	0.0	0.0	0.285609	105040	105670	694-701	0.888889	75.3
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	GGGGCAACAACAACAAACAA	GCTTGAGCACTGCTTCCTAA	20	20	45.0	50.0	57.9	58.2	0.0	4.7	0.287091	105040	105670	715-722	0.888889	75.3
ccsA_p1	ccsA	1	CTCAAGCATCTCGGTTCGAA	TGAGTGGTTTTGTCGCAGAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.034967	105828	106787	1570-1574	1.000000	81.1
ccsA_p2	ccsA	2	CTCAAGCATCTCGGTTCGAA	GCCTTACCAGGAATGAGTGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.047831	105828	106787	1583-1587	1.000000	81.0
ccsA_p3	ccsA	3	CTCAAGCATCTCGGTTCGAA	ATCTCGAAGAACTTGGGGGA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.051087	105828	106787	1663-1667	1.000000	80.9
ccsA_p4	ccsA	4	AAGCATCTCGGTTCGAATCC	TGAGTGGTTTTGTCGCAGAA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.089933	105828	106787	1567-1571	1.000000	80.7
ccsA_p5	ccsA	5	AAGCATCTCGGTTCGAATCC	GCCTTACCAGGAATGAGTGG	20	20	50.0	55.0	58.1	58.0	0.0	0.0	0.102798	105828	106787	1580-1584	1.000000	80.5