Hotspot

trnL-UAG-rpl32

Genome · 128687-129407 · 721 bp

Back to Austrocallerya

Pi 0.0274
MarkerSeek score 64.60
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
Genome
length_bp
721
variable_sites
19
pi
0.0274
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 TCCTAAGAGCAGCGTGTCTA TGGACAGCATTGAAAGCCTT 50.0 45.0 57.9 58.0 839-841 bp 1.0 83.5
trnL-UAG-rpl32_p2 GAAGTAGCAAAGCCTTGGGT TGGACAGCATTGAAAGCCTT 50.0 45.0 58.1 58.0 1350-1353 bp 1.0 82.5
trnL-UAG-rpl32_p3 GAAGTAGCAAAGCCTTGGGT TTGGACAGCATTGAAAGCCT 50.0 45.0 58.1 58.0 1351-1354 bp 1.0 82.5
trnL-UAG-rpl32_p4 ATGGGATTGTAGAGCGGGTA TGGACAGCATTGAAAGCCTT 50.0 45.0 57.9 58.0 1408-1411 bp 1.0 82.3
trnL-UAG-rpl32_p5 ATGGGATTGTAGAGCGGGTA TTGGACAGCATTGAAAGCCT 50.0 45.0 57.9 58.0 1409-1412 bp 1.0 82.3