Hotspot

ycf1-rps15

Genome · 118553-119053 · 501 bp

Back to Austrocallerya

Pi 0.0187

MarkerSeek score 64.90

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: Genome
length_bp: 501
variable_sites: 9
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	ACCCATAATGGAAGCCGTTT	TCAACTGATCCGGTGTTTTCT	45.0	42.9	57.5	57.5	616-635 bp	1.0	67.5
ycf1-rps15_p2	ACCCATAATGGAAGCCGTTT	ACTGATCCGGTGTTTTCTTCT	45.0	42.9	57.5	57.2	613-632 bp	1.0	65.2
ycf1-rps15_p3	ACCCATAATGGAAGCCGTTT	TCAACTGATCCGGTGTTTTC	45.0	45.0	57.5	56.0	616-635 bp	1.0	63.3
ycf1-rps15_p4	ACCCATAATGGAAGCCGTTT	CAACTGATCCGGTGTTTTCT	45.0	45.0	57.5	56.0	615-634 bp	1.0	63.2
ycf1-rps15_p5	ACCCATAATGGAAGCCGTTT	ACTGATCCGGTGTTTTCTTCTT	45.0	40.9	57.5	57.9	613-632 bp	1.0	62.7