Hotspot

trnN-GUU-ycf1

Genome · 112605-113263 · 659 bp

Back to Austrocallerya

Pi 0.0409

MarkerSeek score 61.80

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: Genome
length_bp: 659
variable_sites: 17
pi: 0.0409
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.63
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	CGGGAGAATTGATGACTGCA	CCCGAGTTCGAAGAAGGAAG	50.0	55.0	58.0	58.0	929-1166 bp	1.0	85.1
trnN-GUU-ycf1_p2	CGGGAGAATTGATGACTGCA	CCGACTACAACGACCGAATT	50.0	50.0	58.0	58.0	861-1098 bp	1.0	85.1
trnN-GUU-ycf1_p3	TTTAAATAGCCCCGGACTGC	CCCGAGTTCGAAGAAGGAAG	50.0	55.0	58.0	58.0	851-1088 bp	1.0	85.1
trnN-GUU-ycf1_p4	CGGGAGAATTGATGACTGCA	GCCCTATGGAGAATGTGGTG	50.0	55.0	58.0	58.0	899-1136 bp	1.0	85.0
trnN-GUU-ycf1_p5	CGGGAGAATTGATGACTGCA	ATGAACGTCATGAGCTGTCC	50.0	50.0	58.0	58.0	1002-1239 bp	1.0	84.9