Hotspot

trnR-ACG-trnN-GUU

Genome · 111965-112532 · 568 bp

Back to Austrocallerya

Pi 0.0158

MarkerSeek score 65.20

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: Genome
length_bp: 568
variable_sites: 9
pi: 0.0158
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	709-723 bp	1.0	79.3
trnR-ACG-trnN-GUU_p2	TACTCCGGGAAACAGATCCA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	57.8	58.0	755-769 bp	1.0	79.0
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	660-674 bp	1.0	78.1
trnR-ACG-trnN-GUU_p4	TACTCCGGGAAACAGATCCA	TGGTCGTAGGTTCGAATCCT	50.0	50.0	57.8	58.2	706-720 bp	1.0	77.8
trnR-ACG-trnN-GUU_p5	GGGAAACAGATCCAGTGGAG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.6	58.0	749-763 bp	1.0	77.7