Hotspot

trnI-CAU-ycf2

Genome · 91248-91335 · 88 bp

Back to Austrocallerya

Pi 0.0455

MarkerSeek score 68.10

Primer available yes

Label trnI-CAU-ycf2

Feature Metadata

label_name: trnI-CAU-ycf2
feature_type: igs
region: Genome
length_bp: 88
variable_sites: 4
pi: 0.0455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-ycf2_p1	GTTGGGCGCTTTAACCATTC	CAAGATTGGAATTCATGTTCC	50.0	38.1	58.0	53.1	146 bp	1.0	42.2
trnI-CAU-ycf2_p2	GAACCTACGACTTCGCCAAT	GGAATTCATGTTCCGTCAT	50.0	42.1	58.0	53.0	164 bp	1.0	42.0
trnI-CAU-ycf2_p3	GAACCTACGACTTCGCCAAT	CAAGATTGGAATTCATGTTCC	50.0	38.1	58.0	53.1	171 bp	1.0	41.9
trnI-CAU-ycf2_p4	ATTGAACCTACGACTTCGCC	CAAGATTGGAATTCATGTTCC	50.0	38.1	58.0	53.1	174 bp	1.0	41.8
trnI-CAU-ycf2_p5	CACTTTTGGAGGGAGGGTTC	CAAGATTGGAATTCATGTTCC	55.0	38.1	58.1	53.1	216 bp	1.0	41.5