Hotspot

atpA-trnR-UCU

Genome · 54419-54601 · 183 bp

Back to Austrocallerya

Pi 0.0393

MarkerSeek score 70.30

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: Genome
length_bp: 183
variable_sites: 7
pi: 0.0393
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	CATTCACTGGGGAAGCAGAA	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	294-297 bp	1.0	68.1
atpA-trnR-UCU_p2	AAGACATTCACTGGGGAAGC	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	298-301 bp	1.0	68.0
atpA-trnR-UCU_p3	GGAAGCAGAAGCCCTTTTGA	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.4	57.3	284-287 bp	1.0	66.8
atpA-trnR-UCU_p4	ATTCACTGGGGAAGCAGAAG	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.5	57.3	293-296 bp	1.0	65.7
atpA-trnR-UCU_p5	GACATTCACTGGGGAAGCAG	GGTTCAAATCCTATTGGACGC	55.0	47.6	58.5	57.3	296-299 bp	1.0	65.3