Hotspot

trnG-GCC-psbZ

Genome · 26247-27089 · 843 bp

Back to Austrocallerya

Pi 0.0132

MarkerSeek score 56.00

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: Genome
length_bp: 843
variable_sites: 10
pi: 0.0132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	ACCATTCGACTATATCCGC	CTTCTCCTGATGGTTGGTCG	47.4	55.0	54.3	58.0	888-969 bp	1.0	46.8
trnG-GCC-psbZ_p2	ACCATTCGACTATATCCGC	TTGCTTCTCCTGATGGTTGG	47.4	50.0	54.3	57.8	891-972 bp	1.0	45.3
trnG-GCC-psbZ_p3	ACCATTCGACTATATCCGC	TCCTGATGGTTGGTCGAGTA	47.4	50.0	54.3	57.8	884-965 bp	1.0	45.1
trnG-GCC-psbZ_p4	ACCATTCGACTATATCCGC	GCTTCTCCTGATGGTTGGTC	47.4	55.0	54.3	58.3	889-970 bp	1.0	44.9
trnG-GCC-psbZ_p5	ACCATTCGACTATATCCGC	TTCTCCTGATGGTTGGTCGA	47.4	50.0	54.3	58.4	887-968 bp	1.0	44.9