Hotspot

ndhK-ndhC

LSC · 103765-103814 · 50 bp

Back to Australopyrum

Pi 0.04

MarkerSeek score 63.90

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 50
variable_sites: 2
pi: 0.04
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	ACCGTATAGAAGGGGCCATA	AAAGGAGCCTTGGAATGGTC	50.0	50.0	57.3	57.8	194 bp	1.0	73.4
ndhK-ndhC_p2	ACCGTATAGAAGGGGCCATAA	AAAGGAGCCTTGGAATGGTC	47.6	50.0	58.0	57.8	194 bp	1.0	70.9
ndhK-ndhC_p3	ACCGTATAGAAGGGGCCATA	AAGGAGCCTTGGAATGGTCT	50.0	50.0	57.3	58.6	193 bp	1.0	70.2
ndhK-ndhC_p4	ACCGTATAGAAGGGGCCATA	AGGAGCCTTGGAATGGTCTT	50.0	50.0	57.3	58.6	192 bp	1.0	70.2
ndhK-ndhC_p5	ACCGTATAGAAGGGGCCATA	AAAAGGAGCCTTGGAATGGT	50.0	45.0	57.3	57.3	195 bp	1.0	69.5