Hotspot

trnR-UCU-rps14

LSC · 90239-90617 · 379 bp

Back to Australopyrum

Pi 0.0211

MarkerSeek score 66.30

Primer available yes

Label trnR-UCU-rps14

Feature Metadata

label_name: trnR-UCU-rps14
feature_type: igs
region: LSC
length_bp: 379
variable_sites: 8
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-rps14_p1	AGAAGACCTCTGTCCTATCCA	TATCCGGACACGTACTTCGA	47.6	50.0	56.9	58.0	486 bp	1.0	64.7
trnR-UCU-rps14_p2	AGAAGACCTCTGTCCTATCCA	CATTTTGGGCTATCCGGACA	47.6	50.0	56.9	57.9	496 bp	1.0	64.5
trnR-UCU-rps14_p3	AGAAGACCTCTGTCCTATCCA	TCGACATTTTGGGCTATCCG	47.6	50.0	56.9	58.1	500 bp	1.0	64.5
trnR-UCU-rps14_p4	AGAAGACCTCTGTCCTATCCA	ACATTTTGGGCTATCCGGAC	47.6	50.0	56.9	57.9	497 bp	1.0	64.5
trnR-UCU-rps14_p5	AGAAGACCTCTGTCCTATCCA	ATTTTGGGCTATCCGGACAC	47.6	50.0	56.9	57.9	495 bp	1.0	64.5