Hotspot

trnC-GCA-rpoB

LSC · 72591-73749 · 1159 bp

Back to Australopyrum

Pi 0.0104

MarkerSeek score 61.60

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1159
variable_sites: 12
pi: 0.0104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	ATTCCCTCGTTTCCATTCCG	50.0	50.0	58.1	58.0	1226-1228 bp	1.0	80.0
trnC-GCA-rpoB_p2	AAAGGATTTGCAGTCCCCTG	TGTGGACATTCCCTCGTTTC	50.0	50.0	58.1	57.8	1233-1235 bp	1.0	78.9
trnC-GCA-rpoB_p3	AAAGGATTTGCAGTCCCCTG	CCGGGTATTGTGGACATTCC	50.0	55.0	58.1	58.3	1241-1243 bp	1.0	77.7
trnC-GCA-rpoB_p4	AAAGGATTTGCAGTCCCCTG	CGGGTATTGTGGACATTCCC	50.0	55.0	58.1	58.3	1240-1242 bp	1.0	77.7
trnC-GCA-rpoB_p5	AAAGGATTTGCAGTCCCCTG	AAGTTCTTCTGCCAAGGCTT	50.0	45.0	58.1	57.6	1305-1307 bp	1.0	77.3