Hotspot

petN-trnC-GCA

LSC · 72030-72519 · 490 bp

Back to Australopyrum

Pi 0.0103

MarkerSeek score 62.90

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 490
variable_sites: 5
pi: 0.0103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	AGTCCACTCCTTCCCCATAC	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	598-1063 bp	1.0	71.4
petN-trnC-GCA_p2	GTCCACTCCTTCCCCATACT	AGTTCAAATCTGGGTGCCG	55.0	52.6	58.1	58.1	597-1062 bp	1.0	71.4
petN-trnC-GCA_p3	AGCCCAAGCGAGACTTACTA	AGTTCAAATCTGGGTGCCG	50.0	52.6	58.2	58.1	539-1004 bp	1.0	71.2
petN-trnC-GCA_p4	AGTCCACTCCTTCCCCATAC	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	600-1065 bp	1.0	70.9
petN-trnC-GCA_p5	GTCCACTCCTTCCCCATACT	CCAGTTCAAATCTGGGTGCC	55.0	55.0	58.1	59.1	599-1064 bp	1.0	70.9