Hotspot

matK-trnK-UUU

LSC · 57630-58310 · 681 bp

Back to Australopyrum

Pi 0.00441

MarkerSeek score 61.30

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 681
variable_sites: 3
pi: 0.00441
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCTGAAAGAGAAGTGGGT	AATGTTTTGACAGAAGCGCG	47.6	45.0	58.3	58.0	875 bp	1.0	69.5
matK-trnK-UUU_p2	ACTCCTGAAAGAGAAGTGGG	AATGTTTTGACAGAAGCGCG	50.0	45.0	56.5	58.0	875 bp	1.0	67.7
matK-trnK-UUU_p3	CTCCTGAAAGAGAAGTGGGT	AATGTTTTGACAGAAGCGCG	50.0	45.0	56.5	58.0	874 bp	1.0	67.7
matK-trnK-UUU_p4	TCCTGAAAGAGAAGTGGGTAGA	AATGTTTTGACAGAAGCGCG	45.5	45.0	57.9	58.0	873 bp	1.0	63.0
matK-trnK-UUU_p5	ACTCCTGAAAGAGAAGTGGGTA	AATGTTTTGACAGAAGCGCG	45.5	45.0	58.1	58.0	875 bp	1.0	62.8