Hotspot

rrn5-rrn4.5

IRa · 132975-133198 · 224 bp

Back to Aulacospermum

Pi 0.0938

MarkerSeek score 82.10

Primer available yes

Label rrn5-rrn4.5

Feature Metadata

label_name: rrn5-rrn4.5
feature_type: igs
region: IRa
length_bp: 224
variable_sites: 21
pi: 0.0938
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn5-rrn4.5_p1	TATCGTCACCGCAGTAGAGT	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	339-373 bp	1.0	84.1
rrn5-rrn4.5_p2	AGTATCGTCACCGCAGTAGA	CAGCTGAGGCATCCTAACAG	50.0	55.0	58.0	58.0	341-375 bp	1.0	84.0
rrn5-rrn4.5_p3	TATCGTCACCGCAGTAGAGT	GCTGAGGCATCCTAACAGAC	50.0	55.0	58.0	58.1	337-371 bp	1.0	84.0
rrn5-rrn4.5_p4	TATCGTCACCGCAGTAGAGT	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	365-399 bp	1.0	83.8
rrn5-rrn4.5_p5	AGTATCGTCACCGCAGTAGA	GTCAAGTGGAAGTGCAGTGA	50.0	50.0	58.0	58.0	367-401 bp	1.0	83.7