Hotspot

ndhF-rpl32

SSC · 114140-115339 · 1200 bp

Back to Aulacospermum

Pi 0.0832

MarkerSeek score 67.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1200
variable_sites: 51
pi: 0.0832
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.51
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGCAAACAATAGAACGGCAC	AATTCCAGGCTTTTGTCGGT	50.0	45.0	58.0	58.0	2017-2597 bp	1.0	88.4
ndhF-rpl32_p2	GGCAAACAATAGAACGGCAC	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	2016-2596 bp	1.0	88.4
ndhF-rpl32_p3	CCGTGTGAACCAAAACCCTA	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	1809-2389 bp	1.0	88.3
ndhF-rpl32_p4	CCCCAACGCGATTAGTTACA	AATTCCAGGCTTTTGTCGGT	50.0	45.0	58.0	58.0	2442 bp	0.5	59.9
ndhF-rpl32_p5	CCCCAACGCGATTAGTTACA	ATTCCAGGCTTTTGTCGGTT	50.0	45.0	58.0	58.0	2441 bp	0.5	59.9