Hotspot

accD-psaI

LSC · 59822-60472 · 651 bp

Back to Aulacospermum

Pi 0.11

MarkerSeek score 81.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 651
variable_sites: 57
pi: 0.11
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCACGCTTTCTTTCCTTTGA	AAAGAAGCCATTGCCATTGC	42.9	45.0	58.1	57.9	689-772 bp	1.0	80.8
accD-psaI_p2	TCCACGCTTTCTTTCCTTTGA	TTGCCATTGCCGGAAATACT	42.9	45.0	58.1	58.2	679-762 bp	1.0	80.5
accD-psaI_p3	CCACGCTTTCTTTCCTTTGA	AAAGAAGCCATTGCCATTGC	45.0	45.0	56.6	57.9	688-771 bp	1.0	77.9
accD-psaI_p4	TCCACGCTTTCTTTCCTTTG	AAAGAAGCCATTGCCATTGC	45.0	45.0	56.6	57.9	689-772 bp	1.0	77.9
accD-psaI_p5	CCACGCTTTCTTTCCTTTGA	TTGCCATTGCCGGAAATACT	45.0	45.0	56.6	58.2	678-761 bp	1.0	77.6