Hotspot

trnF-GAA-ndhJ

LSC · 49748-50151 · 404 bp

Back to Aulacospermum

Pi 0.0878
MarkerSeek score 81.50
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
404
variable_sites
33
pi
0.0878
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA CGTAAGGATTATATTGCCCCCA 55.0 45.5 58.0 57.7 499-525 bp 1.0 70.8
trnF-GAA-ndhJ_p2 GGGATAGCTCAGCTGGTAGA ACGTAAGGATTATATTGCCCCC 55.0 45.5 58.0 57.7 500-526 bp 1.0 70.8
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA CGTAAGGATTATATTGCCCCCA 50.0 45.5 58.1 57.7 463-489 bp 1.0 70.8
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA ACGTAAGGATTATATTGCCCCC 50.0 45.5 58.1 57.7 464-490 bp 1.0 70.7
trnF-GAA-ndhJ_p5 CTGGTAGAGCAGAGGACTGA CGTAAGGATTATATTGCCCCCA 55.0 45.5 57.9 57.7 487-513 bp 1.0 69.8