Hotspot

trnE-UUC-trnT-GGU

LSC · 31664-32487 · 824 bp

Back to Aulacospermum

Pi 0.15

MarkerSeek score 81.20

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 824
variable_sites: 107
pi: 0.15
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GGCGAAACGAAAAGGGAAAG	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1342-1399 bp	1.0	91.6
trnE-UUC-trnT-GGU_p2	CAATCCCGGGGAAATAAGGG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	1222 bp	0.5	59.9
trnE-UUC-trnT-GGU_p3	TGAGAAGGGCGAAACGAAAA	GAACCGATGACTTACGCCTT	45.0	50.0	58.0	58.0	1406 bp	0.5	59.6
trnE-UUC-trnT-GGU_p4	CAATCCCGGGGAAATAAGGG	TGGTACATGAAGCTGCGATC	55.0	50.0	58.0	58.1	1902 bp	0.5	59.5
trnE-UUC-trnT-GGU_p5	CAATCCCGGGGAAATAAGGG	TGGTGATTCCAGAGACGACT	55.0	50.0	58.0	58.1	1489 bp	0.5	59.4