Hotspot

trnC-GCA-petN

LSC · 28622-29230 · 609 bp

Back to Aulacospermum

Pi 0.0678
MarkerSeek score 83.80
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
609
variable_sites
39
pi
0.0678
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 665-757 bp 1.0 86.3
trnC-GCA-petN_p2 CGATATGGCCGAGTGGTAAG AGCCCAAGCGAGACTTACTA 55.0 50.0 57.9 58.2 705-797 bp 1.0 86.0
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 683-775 bp 1.0 84.0
trnC-GCA-petN_p4 GATATGGCCGAGTGGTAAGG AGCCCAAGCGAGACTTACTA 55.0 50.0 57.5 58.2 704-796 bp 1.0 82.9
trnC-GCA-petN_p5 CGATATGGCCGAGTGGTAAG GCCCAAGCGAGACTTACTAT 55.0 50.0 57.9 57.1 704-796 bp 1.0 80.1