Hotspot

rpoB-trnC-GCA

LSC · 27330-28550 · 1221 bp

Back to Aulacospermum

Pi 0.0761

MarkerSeek score 79.50

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1221
variable_sites: 90
pi: 0.0761
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGAGCCCATATCCTTGCTTT	GATCGGGAAGTCGTGATAGC	50.0	55.0	58.0	57.9	1980-2006 bp	1.0	85.6
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	CTTACCACTCGGCCATATCG	50.0	55.0	58.0	57.9	1755-1775 bp	1.0	85.5
rpoB-trnC-GCA_p3	CCGCCTACACAAGCAAATTG	GATCGGGAAGTCGTGATAGC	50.0	55.0	58.0	57.9	2172-2198 bp	1.0	85.2
rpoB-trnC-GCA_p4	CCGCCTACACAAGCAAATTG	CTTACCACTCGGCCATATCG	50.0	55.0	58.0	57.9	1947-1967 bp	1.0	85.1
rpoB-trnC-GCA_p5	CCGCCTACACAAGCAAATTG	TAGAGTGGGATCGGGAAGTC	50.0	55.0	58.0	57.9	2180 bp	0.5	59.3