Hotspot

rps16-trnQ-UUG

LSC · 6314-7364 · 1051 bp

Back to Aulacospermum

Pi 0.2
MarkerSeek score 84.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1051
variable_sites
194
pi
0.2
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TTCTACCCGAGCTCCATCAT TTTCTCGCAGCTATCCAACC 50.0 50.0 57.9 58.0 2000-2323 bp 1.0 90.3
rps16-trnQ-UUG_p2 AAAGGGCGTTTCCTTGTTCT TTTCTCGCAGCTATCCAACC 45.0 50.0 57.9 58.0 2352 bp 0.5 59.3
rps16-trnQ-UUG_p3 AAAGGGCGTTTCCTTGTTCT CGCAGCTATCCAACCAAGAT 45.0 50.0 57.9 58.0 2347 bp 0.5 59.1
rps16-trnQ-UUG_p4 AAAGGGCGTTTCCTTGTTCT CTTACAGCAGCTTGCCAAAC 45.0 50.0 57.9 57.9 2209 bp 0.5 59.0
rps16-trnQ-UUG_p5 TTCTACCCGAGCTCCATCAT CGCAGCTATCCAACCAAGAT 50.0 50.0 57.9 58.0 1995 bp 0.5 59.0