Hotspot

trnK-UUU-rps16

LSC · 4285-5183 · 899 bp

Back to Aulacospermum

Pi 0.103
MarkerSeek score 68.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
899
variable_sites
55
pi
0.103
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.60
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCAGGATCAGTCGTGG TCTATCCCAATGAGCCGTCT 55.0 50.0 58.0 57.9 880-1236 bp 1.0 88.1
trnK-UUU-rps16_p2 CCCTTTCAGGATCAGTCGTG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 941-1297 bp 1.0 87.9
trnK-UUU-rps16_p3 CCTTTCAGGATCAGTCGTGG GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 940-1296 bp 1.0 87.9
trnK-UUU-rps16_p4 CCCTTTCAGGATCAGTCGTG AGAACAAGGAAACGCCCTTT 55.0 45.0 58.0 57.9 1625 bp 0.5 59.3
trnK-UUU-rps16_p5 CCTTTCAGGATCAGTCGTGG AGAACAAGGAAACGCCCTTT 55.0 45.0 58.0 57.9 1624 bp 0.5 59.3