Hotspot

ycf1

SSC · 127448-133123 · 5676 bp

Pi 0.00757

MarkerSeek score 39.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5676
variable_sites: 108
pi: 0.00757
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCACTCGGGAAGTTGCAATA	TCCGCTCAGTTTGTCAACTT	50.0	45.0	57.9	57.7	1312-1327 bp	1.0	79.0
ycf1_p2	AATGGAATGGTATCCCACGC	TCCGCTCAGTTTGTCAACTT	50.0	45.0	58.0	57.7	1188-1203 bp	0.8	71.1
ycf1_p3	AATGGAATGGTATCCCACGC	AAAGACCGCTTCTGGTTTGA	50.0	45.0	58.0	57.7	1367-1382 bp	0.8	70.9
ycf1_p4	AATGGAATGGTATCCCACGC	AAGACCGCTTCTGGTTTGAA	50.0	45.0	58.0	57.7	1366-1381 bp	0.8	70.9
ycf1_p5	AATGGAATGGTATCCCACGC	AGACCGCTTCTGGTTTGAAA	50.0	45.0	58.0	57.7	1365-1380 bp	0.8	70.9