Hotspot

rps16

LSC · 5083-6214 · 1132 bp

Back to Aucuba

Pi 0.0105
MarkerSeek score 40.40
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1132
variable_sites
23
pi
0.0105
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 ACTTGTGTTGGATTGGCACT GCAGATTCCCAAAGAGCTCA 45.0 50.0 57.9 57.9 1826-1864 bp 1.0 80.6
rps16_p2 ACTTGTGTTGGATTGGCACT TGCAGATTCCCAAAGAGCTC 45.0 50.0 57.9 57.9 1827-1865 bp 1.0 80.6
rps16_p3 TCCAACGAAAACCACCCAAT GCAGATTCCCAAAGAGCTCA 45.0 50.0 57.9 57.9 1659-1697 bp 1.0 80.6
rps16_p4 TCCAACGAAAACCACCCAAT TGCAGATTCCCAAAGAGCTC 45.0 50.0 57.9 57.9 1660-1698 bp 1.0 80.6
rps16_p5 TTCCAACGAAAACCACCCAA GCAGATTCCCAAAGAGCTCA 45.0 50.0 58.2 57.9 1660-1698 bp 1.0 80.0