Hotspot

rpoC2

LSC · 17740-21912 · 4173 bp

Pi 0.00267

MarkerSeek score 51.20

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4173
variable_sites: 30
pi: 0.00267
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	TAAAGTGGATGTTCCCGCAC	TATTCACAGGGGGTACTGCA	50.0	50.0	58.2	58.0	263 bp	1.0	79.7
rpoC2_p2	TAAAGTGGATGTTCCCGCAC	ATTGGAGAACCGGGTACTCA	50.0	50.0	58.2	58.1	315 bp	1.0	79.4
rpoC2_p3	TAAAGTGGATGTTCCCGCAC	TGGAGAACCGGGTACTCAAT	50.0	50.0	58.2	58.1	313 bp	1.0	79.4
rpoC2_p4	TAAAGTGGATGTTCCCGCAC	ACTGCAGAACATGTGAGAGC	50.0	50.0	58.2	58.2	249 bp	1.0	78.6
rpoC2_p5	TAAAGTGGATGTTCCCGCAC	CGGAGTATTCACAGGGGGTA	50.0	55.0	58.2	58.2	268 bp	1.0	78.3