Hotspot

ndhA

SSC · 123309-125513 · 2205 bp

Pi 0.00531

MarkerSeek score 37.80

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2205
variable_sites: 29
pi: 0.00531
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATTCAGCCACGATTCTCACC	ATGGGGGAAGTTGATCGTTG	50.0	50.0	58.0	57.9	2937-2948 bp	1.0	79.9
ndhA_p2	ATTCAGCCACGATTCTCACC	GGGGGAAGTTGATCGTTGAA	50.0	50.0	58.0	57.8	2935-2946 bp	1.0	79.4
ndhA_p3	ACGATTCGCATGGGATAAGG	ATGGGGGAAGTTGATCGTTG	50.0	50.0	57.8	57.9	2412-2423 bp	1.0	78.9
ndhA_p4	CGATTCGCATGGGATAAGGT	ATGGGGGAAGTTGATCGTTG	50.0	50.0	57.8	57.9	2411-2422 bp	1.0	78.9
ndhA_p5	ACCAATGTACCTTGCAGCTC	ATGGGGGAAGTTGATCGTTG	50.0	50.0	58.2	57.9	2376-2387 bp	1.0	78.9