Hotspot

ndhC-trnV-UAC

LSC · 53762-54816 · 1055 bp

Back to Aucuba

Pi 0.00449
MarkerSeek score 51.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1055
variable_sites
12
pi
0.00449
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AATCGGGGCTAAAATTCCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 1182-1191 bp 1.0 78.5
ndhC-trnV-UAC_p2 CTGGTCCTTTGCTAATCGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.3 57.8 1195-1204 bp 1.0 78.4
ndhC-trnV-UAC_p3 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1190-1199 bp 1.0 78.3
ndhC-trnV-UAC_p4 TCCTTTGCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.5 57.8 1191-1200 bp 1.0 77.0
ndhC-trnV-UAC_p5 CGGGGCTAAAATTCCGGAAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.5 57.8 1179-1188 bp 1.0 76.8