Hotspot

ycf3-trnS-GGA

LSC · 47275-48127 · 853 bp

Back to Aucuba

Pi 0.00753
MarkerSeek score 56.20
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
853
variable_sites
17
pi
0.00753
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 CATTTATCCGCGATCTAGGCA CCCTCGGTAAACAAAAGCCT 47.6 50.0 58.0 58.1 939-953 bp 1.0 73.0
ycf3-trnS-GGA_p2 CATTTATCCGCGATCTAGGCA ACGGAAAGAGAGGGATTCGA 47.6 50.0 58.0 58.2 960-974 bp 1.0 72.5
ycf3-trnS-GGA_p3 CATTTATCCGCGATCTAGGCA TTCCAATGCTACGCCTTGAA 47.6 45.0 58.0 57.8 910-924 bp 1.0 72.2
ycf3-trnS-GGA_p4 CATTTATCCGCGATCTAGGCA ACCCTCGGTAAACAAAAGCC 47.6 50.0 58.0 58.4 940-954 bp 1.0 70.6
ycf3-trnS-GGA_p5 TTTATCCGCGATCTAGGCAT CCCTCGGTAAACAAAAGCCT 45.0 50.0 56.6 58.1 937-951 bp 1.0 69.9