Hotspot

psaA-ycf3

LSC · 44415-45269 · 855 bp

Back to Aucuba

Pi 0.00491
MarkerSeek score 56.80
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
855
variable_sites
10
pi
0.00491
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.93
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 CCCATTCCTCGAAAGACGTT ATCAAGCCGCTGAGTATTGG 50.0 50.0 57.9 58.0 970-1053 bp 1.0 83.7
psaA-ycf3_p2 CCCATTCCTCGAAAGACGTT GGAAACAGGCTATAGCGCTT 50.0 50.0 57.9 58.0 952-1035 bp 1.0 83.7
psaA-ycf3_p3 CCCATTCCTCGAAAGACGTT CGATCAAGCCGCTGAGTATT 50.0 50.0 57.9 58.1 972-1055 bp 1.0 82.9
psaA-ycf3_p4 CCCATTCCTCGAAAGACGTT TTGAAGATCACGAGGCGTTT 50.0 45.0 57.9 57.8 899-982 bp 1.0 82.8
psaA-ycf3_p5 CCCATTCCTCGAAAGACGTT TTGGTTGAAGATCACGAGGC 50.0 50.0 57.9 58.2 903-986 bp 1.0 82.6