Hotspot

psbM-trnD-GUC

LSC · 31541-32723 · 1183 bp

Back to Aucuba

Pi 0.0101
MarkerSeek score 50.80
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1183
variable_sites
33
pi
0.0101
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.8 58.5 1283-1306 bp 1.0 62.7
psbM-trnD-GUC_p2 AGGAACTAGAATGAACAGTGCA TTCAATTGGTCAGAGCACCG 40.9 50.0 57.4 58.5 1290-1313 bp 1.0 59.6
psbM-trnD-GUC_p3 AGAATGAACAGTGCAGTAGC GTTCAATTGGTCAGAGCACC 45.0 50.0 55.8 57.0 1284-1307 bp 1.0 58.6
psbM-trnD-GUC_p4 GGAACTAGAATGAACAGTGCAG TTCAATTGGTCAGAGCACCG 45.5 50.0 57.1 58.5 1289-1312 bp 1.0 57.1
psbM-trnD-GUC_p5 AGAATGAACAGTGCAGTAGC TCAATTGGTCAGAGCACCG 45.0 52.6 55.8 57.8 1282-1305 bp 1.0 56.7