Hotspot

ycf2

IRa · 145689-152528 · 6840 bp

Back to Attalea

Pi 0.0121

MarkerSeek score 46.60

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: IRa
length_bp: 6840
variable_sites: 124
pi: 0.0121
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	AACACAGAGACAAAGGGCTG	TCGGTCTCTTGGTGAACTCT	50.0	50.0	58.0	58.0	1261 bp	0.333333	53.1
ycf2_p2	AACACAGAGACAAAGGGCTG	GTGCACGAGATCTTGTAGCA	50.0	50.0	58.0	58.0	997 bp	0.333333	53.1
ycf2_p3	AACACAGAGACAAAGGGCTG	TGCACGAGATCTTGTAGCAC	50.0	50.0	58.0	58.0	996 bp	0.333333	53.1
ycf2_p4	CTCTGGTACTGCATGGTTCC	TCGGTCTCTTGGTGAACTCT	55.0	50.0	58.0	58.0	1150 bp	0.333333	53.1
ycf2_p5	CTCTGGTACTGCATGGTTCC	TGCACGAGATCTTGTAGCAC	55.0	50.0	58.0	58.0	885 bp	0.333333	53.1