Hotspot

trnL-CAA-ycf2

IRa · 144779-145688 · 910 bp

Back to Attalea

Pi 0.0472

MarkerSeek score 60.20

Primer available yes

Label trnL-CAA-ycf2

Feature Metadata

label_name: trnL-CAA-ycf2
feature_type: igs
region: IRa
length_bp: 910
variable_sites: 53
pi: 0.0472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf2_p1	AAATGGTAGACACGCGAGAC	CAGCCCTTTGTCTCTGTGTT	50.0	50.0	58.0	58.0	1324-1325 bp	1.0	88.1
trnL-CAA-ycf2_p2	TGAAATGGTAGACACGCGAG	CAGCCCTTTGTCTCTGTGTT	50.0	50.0	58.0	58.0	1326-1327 bp	1.0	88.1
trnL-CAA-ycf2_p3	TGCTAAAAAGCGTGGAGGTT	CAGCCCTTTGTCTCTGTGTT	45.0	50.0	58.0	58.0	1293-1294 bp	1.0	88.1
trnL-CAA-ycf2_p4	AAATGGTAGACACGCGAGAC	GGAACCATGCAGTACCAGAG	50.0	55.0	58.0	58.0	1435 bp	0.333333	53.0
trnL-CAA-ycf2_p5	TGAAATGGTAGACACGCGAG	GGAACCATGCAGTACCAGAG	50.0	55.0	58.0	58.0	1437 bp	0.333333	53.0