Hotspot

ccsA-ndhD

SSC · 115482-115697 · 216 bp

Back to Attalea

Pi 0.0154

MarkerSeek score 62.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 216
variable_sites: 5
pi: 0.0154
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACGAATTCGGCACTTATAGCT	TCAGTTGACAGGGTACAAGC	42.9	50.0	57.5	57.5	384 bp	1.0	65.1
ccsA-ndhD_p2	ACGAATTCGGCACTTATAGCT	CAGTTGACAGGGTACAAGCT	42.9	50.0	57.5	57.4	383 bp	1.0	65.0
ccsA-ndhD_p3	ACGAATTCGGCACTTATAGC	TCAGTTGACAGGGTACAAGC	45.0	50.0	56.0	57.5	384 bp	1.0	61.0
ccsA-ndhD_p4	ACGAATTCGGCACTTATAGCT	ATCAGTTGACAGGGTACAAGC	42.9	47.6	57.5	57.9	385 bp	1.0	60.9
ccsA-ndhD_p5	ACGAATTCGGCACTTATAGC	CAGTTGACAGGGTACAAGCT	45.0	50.0	56.0	57.4	383 bp	1.0	60.9