Hotspot

ycf2-trnL-CAA

IRb · 94024-94933 · 910 bp

Back to Attalea

Pi 0.0472

MarkerSeek score 60.20

Primer available yes

Label ycf2-trnL-CAA

Feature Metadata

label_name: ycf2-trnL-CAA
feature_type: igs
region: IRb
length_bp: 910
variable_sites: 53
pi: 0.0472
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnL-CAA_p1	CAGCCCTTTGTCTCTGTGTT	AAATGGTAGACACGCGAGAC	50.0	50.0	58.0	58.0	1324-1325 bp	1.0	88.1
ycf2-trnL-CAA_p2	CAGCCCTTTGTCTCTGTGTT	TGAAATGGTAGACACGCGAG	50.0	50.0	58.0	58.0	1326-1327 bp	1.0	88.1
ycf2-trnL-CAA_p3	CAGCCCTTTGTCTCTGTGTT	TGCTAAAAAGCGTGGAGGTT	50.0	45.0	58.0	58.0	1293-1294 bp	1.0	88.1
ycf2-trnL-CAA_p4	GGAACCATGCAGTACCAGAG	AAATGGTAGACACGCGAGAC	55.0	50.0	58.0	58.0	1435 bp	0.333333	53.0
ycf2-trnL-CAA_p5	GGAACCATGCAGTACCAGAG	TGAAATGGTAGACACGCGAG	55.0	50.0	58.0	58.0	1437 bp	0.333333	53.0