Hotspot

ndhC-trnV-UAC

LSC · 49894-51330 · 1437 bp

Back to Attalea

Pi 0.0028

MarkerSeek score 60.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1437
variable_sites: 6
pi: 0.0028
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTCACTAACCGGTGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	1575-1581 bp	1.0	78.5
ndhC-trnV-UAC_p2	TAACCGGTGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1567-1573 bp	1.0	78.4
ndhC-trnV-UAC_p3	TCCTTCACTAACCGGTGCTA	AGCCCAATGCAACAAAATGC	50.0	45.0	58.1	58.5	1657-1663 bp	1.0	76.2
ndhC-trnV-UAC_p4	TAACCGGTGCTAAAACTCCG	AGCCCAATGCAACAAAATGC	50.0	45.0	57.9	58.5	1649-1655 bp	1.0	76.1
ndhC-trnV-UAC_p5	TCCTTCACTAACCGGTGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	1574-1580 bp	1.0	75.7