Hotspot

ycf1

SSC · 122880-128498 · 5619 bp

Back to Atriplex

Pi 0.0125

MarkerSeek score 46.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5619
variable_sites: 136
pi: 0.0125
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGGGATGAGCTATTCCTTGG	CTGTGGGACTGGAAACTGAC	55.0	55.0	57.8	58.1	990-996 bp	1.0	81.3
ycf1_p2	CGGGATGAGCTATTCCTTGG	AAATGCACCTATAACGGCGT	55.0	45.0	57.8	58.0	1189 bp	0.8	73.5
ycf1_p3	CGGGATGAGCTATTCCTTGG	AATGCACCTATAACGGCGTT	55.0	45.0	57.8	58.0	1188 bp	0.8	73.5
ycf1_p4	CGGGATGAGCTATTCCTTGG	CTGACTTTCCTTTTGGGCCT	55.0	50.0	57.8	58.0	975-981 bp	0.6	62.8
ycf1_p5	CGGGATGAGCTATTCCTTGG	TGACTTTCCTTTTGGGCCTC	55.0	50.0	57.8	58.0	974-980 bp	0.6	62.8