Hotspot

rpl22

LSC · 82930-83526 · 597 bp

Back to Atriplex

Pi 0.0224

MarkerSeek score 56.60

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 597
variable_sites: 27
pi: 0.0224
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CCAAGTCTGAAACCAAGTGGA	AAAGTTCCAGGGACATGCAA	47.6	45.0	58.1	57.6	754 bp	1.0	71.3
rpl22_p2	CCAAGTCTGAAACCAAGTGGA	AAGTTCCAGGGACATGCAAA	47.6	45.0	58.1	57.6	753 bp	1.0	71.3
rpl22_p3	CCAAGTCTGAAACCAAGTGGA	AGTTCCAGGGACATGCAAAA	47.6	45.0	58.1	57.6	752 bp	1.0	71.3
rpl22_p4	ACCAAGTCTGAAACCAAGTGG	AAGTTCCAGGGACATGCAAA	47.6	45.0	58.3	57.6	754 bp	1.0	69.1
rpl22_p5	ACCAAGTCTGAAACCAAGTGG	AGTTCCAGGGACATGCAAAA	47.6	45.0	58.3	57.6	753 bp	1.0	69.1