Hotspot

rpl16

LSC · 80635-82106 · 1472 bp

Back to Atriplex

Pi 0.0101

MarkerSeek score 60.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1472
variable_sites: 26
pi: 0.0101
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCCAAAAGATCCATTTTCCACA	TCAATTCGATTGACCTTTTGA	36.4	33.3	56.1	53.7	1519-1532 bp	1.0	42.2
rpl16_p2	TCCAAAAGATCCATTTTCCACA	TCAATTCGATTGACCTTTTGAA	36.4	31.8	56.1	54.6	1519-1532 bp	1.0	42.2
rpl16_p3	TCCAAAAGATCCATTTTCCACA	TTCCATCGAACAAATTCTCA	36.4	35.0	56.1	52.8	1568-1581 bp	1.0	42.1
rpl16_p4	TCCAAAAGATCCATTTTCCACA	TTTCCATCGAACAAATTCTCA	36.4	33.3	56.1	53.7	1569-1582 bp	1.0	42.1
rpl16_p5	TCCAAAAGATCCATTTTCCACA	TTTTCCATCGAACAAATTCTCA	36.4	31.8	56.1	54.6	1570-1583 bp	1.0	42.1