Hotspot

rpl32-trnL-UAG

SSC · 112257-113314 · 1058 bp

Back to Atriplex

Pi 0.0169

MarkerSeek score 64.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1058
variable_sites: 36
pi: 0.0169
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	ACGAAAAATAAATAACCAAACCTTG	AGCGTGTCTACCGATTTCAC	28.0	50.0	55.3	58.0	944-1118 bp	1.0	46.7
rpl32-trnL-UAG_p2	ACGAAAAATAAATAACCAAACCTTG	CAGCGTGTCTACCGATTTCA	28.0	50.0	55.3	58.0	945-1119 bp	1.0	46.7
rpl32-trnL-UAG_p3	ACGAAAAATAAATAACCAAACCTTG	CTCGCACTGCTTCCTAAGAG	28.0	55.0	55.3	58.1	965-1139 bp	1.0	46.7
rpl32-trnL-UAG_p4	ACGAAAAATAAATAACCAAACCTTG	CTCTCGCACTGCTTCCTAAG	28.0	55.0	55.3	58.1	967-1141 bp	1.0	46.7
rpl32-trnL-UAG_p5	ACGAAAAATAAATAACCAAACCTTG	TCCTAAGAGCAGCGTGTCTA	28.0	50.0	55.3	57.9	954-1128 bp	1.0	46.7