Hotspot

trnP-UGG-psaJ

LSC · 65987-66420 · 434 bp

Back to Atriplex

Pi 0.012

MarkerSeek score 58.00

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 434
variable_sites: 9
pi: 0.012
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TGCCACGGAAAGGTATGTTT	50.0	45.0	58.1	57.7	505-522 bp	1.0	81.0
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TGCCACGGAAAGGTATGTTT	50.0	45.0	58.1	57.7	504-521 bp	1.0	81.0
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	TGCCACGGAAAGGTATGTTT	50.0	45.0	58.1	57.7	516-533 bp	1.0	80.9
trnP-UGG-psaJ_p4	ACCCGTGACATTTTGTACCC	CCAGCTAAAGACCCAAACCA	50.0	50.0	58.1	57.7	543-560 bp	1.0	80.7
trnP-UGG-psaJ_p5	CCCGTGACATTTTGTACCCA	CCAGCTAAAGACCCAAACCA	50.0	50.0	58.1	57.7	542-559 bp	1.0	80.7