Hotspot

ycf4-cemA

LSC · 59872-60469 · 598 bp

Back to Atriplex

Pi 0.0214

MarkerSeek score 62.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 598
variable_sites: 27
pi: 0.0214
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGTGTACCAATTGAAGGA	CCACCAAGTAGTAACCCAAGA	45.0	47.6	57.4	56.9	741-746 bp	1.0	62.0
ycf4-cemA_p2	TGCGTGTACCAATTGAAGGA	TCCACCAAGTAGTAACCCAAG	45.0	47.6	57.4	56.9	742-747 bp	1.0	62.0
ycf4-cemA_p3	TGCGTGTACCAATTGAAGGA	TCCACCAAGTAGTAACCCAA	45.0	45.0	57.4	55.7	742-747 bp	1.0	59.9
ycf4-cemA_p4	TGCGTGTACCAATTGAAGGA	TTCCACCAAGTAGTAACCCA	45.0	45.0	57.4	55.7	743-748 bp	1.0	59.9
ycf4-cemA_p5	TGCGTGTACCAATTGAAGGA	TTCCACCAAGTAGTAACCCAAG	45.0	45.5	57.4	57.6	743-748 bp	1.0	59.6