Hotspot

trnS-GCU-trnG-UCC

LSC · 7729-8437 · 709 bp

Back to Atriplex

Pi 0.00959

MarkerSeek score 63.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 13
pi: 0.00959
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	AGGAAACGGAAAGAGAGGGA	TTAATCAAACCGGCAGACCC	50.0	50.0	58.0	58.2	808-888 bp	1.0	83.6
trnS-GCU-trnG-UCC_p2	ACGGAAAGAGAGGGATTCGA	TTAATCAAACCGGCAGACCC	50.0	50.0	58.2	58.2	803-883 bp	1.0	82.6
trnS-GCU-trnG-UCC_p3	CGAATGACTCGTACAACGGA	TTAATCAAACCGGCAGACCC	50.0	50.0	57.7	58.2	773-853 bp	1.0	81.9
trnS-GCU-trnG-UCC_p4	CTTTCGTCCACTCAGCCATC	TTAATCAAACCGGCAGACCC	55.0	50.0	58.6	58.2	739-819 bp	1.0	78.8
trnS-GCU-trnG-UCC_p5	AACGGAAAGAGAGGGATTCG	TTAATCAAACCGGCAGACCC	50.0	50.0	57.3	58.2	804-884 bp	1.0	78.4